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Conferences > ECBO > 2015 > PSVI > 954216
  • Medical Laser Applications and Laser-Tissue Interactions VII
  • SPIE Proceedings (Optica Publishing Group, 2015),
  • paper 954216

Histological study of subcutaneous fat at NIR laser treatment of the rat skin in vivo

I. Yu. Yanina, Yu. I. Svenskaya, N. A. Navolokin, O. V. Matveeva, A. B. Bucharskaya, G. N. Мaslyakova, D. A. Gorin, G. B. Sukhorukov, and V. V. Tuchin

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Abstract

The goal of this work is to quantify impact of in vivo photochemical treatment using indocyanine green (ICG) or encapsulated ICG and NIR laser irradiation through skin of rat with obesity by the follow up tissue sampling and histochemistry. After 1 hour elapsed since 1-min light exposure samples of rat skin with subcutaneous tissue of thickness of 1.5-2.5 mm were taken by surgery from rats within marked 4-zones of the skin site. For hematoxylin-eosin histological examination of excised tissue samples, fixation was carried out by 10%-formaldehyde solution. For ICG and encapsulated ICG subcutaneous injection and subsequent 1-min diode laser irradiation with power density of 8 W/cm2, different necrotic regions with lipolysis of subcutaneous fat were observed. The obtained data can be used for safe layer-by-layer laser treatment of obesity and cellulite.

© 2015 SPIE

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