Optimal Staining and Clearing Protocol for Whole Mouse Brain Vasculature Imaging with Light-sheet Microscopy

Antonino Paolo Di Giovanna; Alessandro Tibo; Ludovico Silvestri; Ludovico Silvestri; Marie Caroline Müllenbroich; Irene Costantini; Leonardo Sacconi; Leonardo Sacconi; Paolo Frasconi; Francesco Saverio Pavone; Francesco Saverio Pavone; Francesco Saverio Pavone

doi:10.1117/12.2285044

Advances in Microscopic Imaging
SPIE Proceedings (Optica Publishing Group, 2017),
paper 104140N
•https://doi.org/10.1117/12.2285044

Optimal Staining and Clearing Protocol for Whole Mouse Brain Vasculature Imaging with Light-sheet Microscopy

Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, and Francesco Saverio Pavone

European Conference on Biomedical Optics 2017

Munich Germany
25–29 June 2017
ISBN: 9781510612860

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Poster Session IV (PSIV)

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A. P. Di Giovanna, A. Tibo, L. Silvestri, M. C. Müllenbroich, I. Costantini, L. Sacconi, P. Frasconi, and F. S. Pavone, "Optimal Staining and Clearing Protocol for Whole Mouse Brain Vasculature Imaging with Light-sheet Microscopy," in Advances in Microscopic Imaging, E. Beaurepaire, ed., Vol. 10414 of SPIE Proceedings (Optica Publishing Group, 2017), paper 104140N.

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Abstract

Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.

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