Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers

Not Accessible

Your library or personal account may give you access

Abstract

Optical tweezers (OT) is a unique Nobel Prize winning technology that has been widely used in studying cell interaction dynamics at a single-cell level with highly accurate manipulating of living cells and the ability to detect ultra-low intercellular forces. The reversible aggregation process of red blood cells (RBCs) that strongly influences the blood rheological properties thus being critical for blood microcirculation has long been research studied. However, in spite of plentiful researches dealing with RBC aggregation behavior based on an average response of a large number of cells, the detailed mechanism and the applicability of the two coexistent yet mutually opposed models to this reversible process require additional information. In this study, a two-channel optical tweezers system is utilized to reveal the influence of the cell interaction time (0-300 sec) on the RBC (dis)aggregation dynamics. The results show that for RBC enforced disaggregation in autologous plasma, the longer the two RBCs adhere to each other, the stronger the intercellular interaction is, and the lower the degree that a certain optical pulling force can separate two cells, whereas no significant effect of cell interaction time on RBC aggregation process was observed. This observation indicates that the RBC aggregation and disaggregation in autologous plasma are governed by different mechanisms and that the hysteresis effect, namely the dependence of the disaggregation force on RBC interaction history, is a significant feature that needs special consideration in researches related to the RBC disaggregation process.

© 2019 SPIE/OSA

PDF Article
More Like This
Combined use of optical tweezers and scanning electron microscopy to reveal influence of nanoparticles on red blood cells interactions

Tatiana Avsievich, Yana Tarakanchikova, Alexey Popov, Artashes Karmenyan, Alexander Bykov, and Igor Meglinski
11075_14 European Conference on Biomedical Optics (ECBO) 2019

Double trap optical tweezers as a tool for direct studying of single red blood cell aggregation

M.D. Khokhlova, A.G. Zhdanov, E.V. Lyubin, S.Y. Rykova, I.A. Sokolova, and A.A. Fedyanin
CTuJJ5 Conference on Lasers and Electro-Optics (CLEO:S&I) 2010

Probing the red blood cell interaction in individual cell pairs by optical tweezers

Ruixue Zhu, Alexey Popov, and Igor Meglinski
AW3I.4 CLEO: Applications and Technology (CLEO:A&T) 2020

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved