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Single blood cell Raman spectroscopy reveals elevated haemoglobin content in poikilocytosis

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Abstract

In this study, confocal Raman spectroscopy revealed biochemical differences between single normal red blood cells and poikilocytes. The intensities of 5 Raman bands associated with oxygenated RBCs are larger in acanthocytes than normal and normal-looking RBCs. Overall, acanthocytes carry more haemoglobin and this is corroborated by CBC and ICP-MS.

© 2019 SPIE/OSA

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