Abstract

Adsorption of photodynamic active dyes (hypericin, chlorin e₆, methylene blue, ALA and fluorescein) and they release from clinoptilolite type of zeolite (CZ) in the presence of biomolecules, such as collagen, albumin and hemoglobin, were quantitatively investigated by absorption and fluorescence spectrometry. Using multiphoton microscopy, we demonstrated that effective inducing of two-photon excited luminescence and second harmonic generation signals in nano/micro-particles of CZ by femtosecond near-infrared laser excitation can be successfully utilized in multiphoton imaging of CZ particles and the dye adsorption processes. In addition, CZ magnetic (MCZ) particles were fabricated and proposed as a promising material for drug delivery and controlled release in biological systems. It is shown that the temperature of the aqueous suspension of MCZ of 2 cm³ can be increased by 7 ºC for 3 minutes by an induction heater. Furthermore, it was demonstrated that MCZ particles have strong inhibition and destruction effects on insulin and lysozyme fibrillization, and the protein amyloid formation is inhibited in a dose-dependent manner. Optical spectrometry and multiphoton microscopy are effective approaches that may reveal potential of magnetic zeolites in drug delivery and biomedical imaging of cancer photodynamic therapy and hyperthermia.

© 2019 SPIE/OSA