Abstract
For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.
© 2019 SPIE/OSA
PDF ArticleMore Like This
Björn Kemper, Luisa Pohl, Mathias Kaiser, Jürgen Schnekenburger, and Steffi Ketelhut
Th1A.4 Digital Holography and Three-Dimensional Imaging (DH) 2019
Björn Kemper, Burkhard Greve, Martin Götte, and Steffi Ketelhut
JW4A.19 3D Image Acquisition and Display: Technology, Perception and Applications (3D) 2016
Chen Litong, Pan Feng, Che Leiping, Cao Runyu, Xiaosu Yi, Zeng Qiang, Tang Zhihui, and Xiao Wen
Th3A.16 Digital Holography and Three-Dimensional Imaging (DH) 2019