Phase transition monitoring in adipose tissue by multiphoton microscope

Irina Yu. Yanina; Irina Yu. Yanina; Viktor V. Nikolaev; Viktor V. Nikolaev; Vyacheslav I. Kochubey; Vyacheslav I. Kochubey; Valery V. Tuchin; Valery V. Tuchin; Valery V. Tuchin; Yuri V. Kistenev; Yuri V. Kistenev

doi:10.1117/12.2526927

Advances in Microscopic Imaging II
SPIE Proceedings (Optica Publishing Group, 2019),
paper 11076_61
•https://doi.org/10.1117/12.2526927

Phase transition monitoring in adipose tissue by multiphoton microscope

Irina Yu. Yanina, Viktor V. Nikolaev, Vyacheslav I. Kochubey, Valery V. Tuchin, and Yuri V. Kistenev

European Conference on Biomedical Optics 2019

Munich Germany
23–25 June 2019
ISBN: 9781510628458

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Poster Session Wednesday - 11076 (PSW11076)

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I. Y. Yanina, V. V. Nikolaev, V. I. Kochubey, V. V. Tuchin, and Y. V. Kistenev, "Phase transition monitoring in adipose tissue by multiphoton microscope," in Advances in Microscopic Imaging II, Vol. EB103 of SPIE Proceedings (Optica Publishing Group, 2019), paper 11076_61.

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Abstract

In the present study, observations of temperature-mediated phase transitions of the components of adipose tissue were done. Pieces of pork abdominal fat were used as samples of adipose tissue. These samples were heated using a temperature-controlled table and observed using a MPTflex two-photon microscope (JenLab GmbH) with fluorescence lifetime imaging microscopy (FLIM) mode. The analysis of FLIM data was done with a software package “Becker&Hickl”. The developed approach has a great potential for obtaining accurate information on the phase-transition processes occurring during metabolism alterations.

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