Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Near-real time monitoring of bacterial viability using the optrode – a portable fluorimeter

Not Accessible

Your library or personal account may give you access

Abstract

A rapid and easy method for the monitoring of both live and dead bacteria in a sample is valuable in many fields of microbiological and pharmacodynamics studies, and for the monitoring of food safety and public health. Efficient, culture-independent detection of live and dead bacteria can be achieved using differentially staining fluorescent dyes SYTO 9 and propidium iodide (PI). Fluorescence microscopy and flow cytometry have been used extensively for detection of these live/dead cell fluorescence signals, however, both these methods require bulky equipment and are relatively expensive to implement.

We are optimising a method to determine live and dead bacterial concentration that takes advantage of an inexpensive fibre-based fluorimeter, the optrode, which can measure fluorescence intensity in bacterial solutions in challenging working environments. The concentrations of live and dead bacteria were predicted using multivariate analysis of optrode-measured fluorescence spectra, which is then compared with results obtained from the flow cytometry measurements. The findings from this study will be used to establish a general method for the monitoring of live and dead bacteria using the optrode.

© 2019 SPIE/OSA

PDF Article
More Like This
Rapid evaluation of bacterial viability using the optrode – a near real time portable fluorimeter

Fang Ou, Cushla McGoverin, Simon Swift, and Frédérique Vanholsbeeck
AW3C.6 Australian Conference on Optical Fibre Technology (ACOFT) 2016

Near-real time evaluation of live and dead bacterial concentration using the optrode - a portable fluorimeter

Fang Ou, Cushla McGoverin, Simon Swift, and Frédérique Vanholsbeeck
JW4A.114 Frontiers in Optics (FiO) 2018

Bacterial cell enumeration using flow cytometry

Fang Ou, Cushla McGoverin, Simon Swift, and Frédérique Vanholsbeeck
W4A.18 Asia-Pacific Optical Sensors Conference (APOS) 2016

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved