QuanTI-FRET Calibration for Quantitative FRET Imaging in Living Samples

Julien LEBLANC; Alexis COULLOMB; Cécile M BIDAN; Fabian WEHNEKAMP; Chen QIAN; Don C LAMB; Aurélie DUPONT

European Conferences on Biomedical Optics 2021 (ECBO)
OSA Technical Digest (Optica Publishing Group, 2021),
paper ES2A.3

QuanTI-FRET Calibration for Quantitative FRET Imaging in Living Samples

Julien LEBLANC, Alexis COULLOMB, Cécile M BIDAN, Fabian WEHNEKAMP, Chen QIAN, Don C LAMB, and Aurélie DUPONT

European Conference on Biomedical Optics 2021

Munich Germany
20–24 June 2021
ISBN: 978-1-943580-95-8

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FLIM and Advanced Fluorescence Techniques (ES2A)

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J. LEBLANC, A. COULLOMB, C. M. BIDAN, F. WEHNEKAMP, C. QIAN, D. C. LAMB, and A. DUPONT, "QuanTI-FRET Calibration for Quantitative FRET Imaging in Living Samples," in European Conferences on Biomedical Optics 2021 (ECBO), OSA Technical Digest (Optica Publishing Group, 2021), paper ES2A.3.

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Abstract

Quantitative FRET imaging in living cells is made possible on a simple fluorescence microscope with this new method. The single-step calibration is robust and gives absolute FRET values, independent of the instrument.

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