Abstract

Three-dimensional (3D) cultured skin with vascular networks is a useful skin substitute that enables rapid perfusion after grafting. However, the efficiency of the medium supply to thick cultured skin is limited, resulting in a reduction in viability. In this study, we applied photobiomodulation (PBM) to control the viability of 3D skins during cultivation. We compared the effects of PBM with illumination by a light-emitting diode (LED) array at four different peak wavelengths (440 nm, 523 nm, 658 nm and 823 nm); PBM was applied once during cultivation and the viability of the 3D skins was evaluated. The results showed that PBM with 823-nm light significantly improved the viability of the skins, while PBM with other wavelengths was not effective. Based on this result, we applied PBM at 823 nm every 24 hours during cultivation, and we found that the viability of the 3D skins with daily PBM was significantly higher than that with single PBM.

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