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Conferences > ECBO > 2023 > AMI > 1263002

Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas B. David, Laure Bally-Cuif, Nicolas Dray, Michel Négrerie, Willy Supatto, and Emmanuel Beaurepaire

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Abstract

We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

© 2023 SPIE

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