Abstract

The recently introduced high-speed multiphoton microscopy technique of Spectro Temporal Laser Imaging by Diffractive Excitation (SLIDE) provides fluorescence microscopy and fluorescence lifetime imaging (FLIM) at kilohertz frame-rates[1]. Combined with flow cytometry, these speeds enable acquisitions of high resolution microscopy images of cells in flow with high sensitivity at specificity. Until now, SLIDE used infrared laser light for nonlinear two-photon imaging. Here we present SLIDE in the Visible by frequency-doubling of the 1064 nm Fourier Domain Mode Locked (FDML) laser [2]. This new one-photon SLIDE system centered around 532 nm was used to image autofluorescent cells using fluorescence and FLIM imaging, as well as acquiring transmission images for a morphological imaging channel. The high-speed and multi-modal acquisition will permit applications in high-throughput cell analysis and cell sorting applications.

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