Abstract
In conventional optoacoustic microscopy, nanosecond pulse duration lasers are employed. When a laser delivering shorter pulse durations is used, it is expected that, from a theoretical point of view, broader, higher-frequency acoustic waves to be generated, therefore a better axial resolution of the instrument. In the present report, this advantage, offered by a picosecond duration pulse laser, to experimentally demonstrate that the axial resolution of an optoacoustic microscopy instrument can be enhanced was exploited. In comparison to a 2 ns pulse duration, an improvement in the axial resolution of ~50% is demonstrated by using excitations with pulses of duration <100 ps. Details of an optoacoustic microscopy instrument, operating at 532 nm, capable to provide high-resolution axial and lateral optoacoustic images, are also presented. The capabilities of the instrument are demonstrated by in-vivo images of Xenopus laevis brain with a similar ~3.8 µm lateral resolution throughout the whole axial imaging range.
© 2023 SPIE
PDF ArticleMore Like This
Yoav Hazan, Ahiad Levi, Michael Nagli, and Amir Rosenthal
126310K European Conference on Biomedical Optics (ECBO) 2023
Gianni Nteroli, Stella Koutsikou, Peter Moselund, Adrian Podoleanu, and Adrian Bradu
JTu3A.99 Frontiers in Optics (FiO) 2019
Johannes Rebling, Héctor Estrada, Sven Gottschalk, and Daniel Razansky
1041505 European Conference on Biomedical Optics (ECBO) 2017