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Optical Tweezers Measure Forces in a Single Biopolymer Molecule

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Microscopic dielectric beads are trapped at a laser beam focus. These beads are then used as handles to mechanically stress single biopolymer molecules and measure their response. Properties such as entropic elasticity, alternate structural states and unfolding/refolding kinetic are observed in single molecules of DNA, RNA or protein.

© 2003 Optical Society of America

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