Abstract
The ability to image and perturb brain tissue that lies deep to the surface of cortex has facilitated the solution of old problems in blood flow dynamics and has begun to provide new insights into neurovascular coupling. We summarize the use of ultra-short laser pulses to probe the structure and function of vasodynamics through two-photon laser scanning microscopy in junction with fluorescent labels of blood serum and tissue. We next describe the emerging use of amplified ultra-short laser pulses for plasma-assisted ablation as a means to perturb either blood flow or neuronal activity deep to the surface. Lastly, we discuss the use of these same tools for high- throughput block-face imaging of histological issue, in particular, the complete reconstruction of cortical vasculature.
© 2008 Optical Society of America
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