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Suppression of Brownian Motion Explores Cooperativity for Single Multi-Subunit Enzymes in Solution

Yan Jiang, Nick Douglas, Nick Conley, Erik Miller, Judith Frydman, and W. E. Moerner

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Abstract

A high-speed Anti-Brownian ELectrokinetic trap enables extended observation of sub-10nm fluorescent objects in solution. For example, single chaperonin enzymes loaded with Cy3-ATP display stepwise photobleaching intensity traces corresponding to the ATP binding/hydrolysis stoichiometry and cooperativity.

© 2010 Optical Society of America

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