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Investigation of 3-D cell-based assays with structured illumination using a digital micromirror device

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Abstract

In this paper, we demonstrate a fluorescence optical detection system with structured illumination using a digital micromirror device to investigate various cell-based assays. To study the performance of the optical detection system, we measured 2-D and 3-D assays of fluorescent microbeads and extended the results with mammalian cells. Through image reconstruction after structured illumination, we have improved fluorescence images by 32 times in terms of the size of axial point-spread function. The findings can be useful to supplement structured illumination microscopy that has been actively investigated for various applications.

© 2012 Optical Society of America

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More Like This
Multimodal approach combining optical diffraction tomography and three-dimensional structured illumination microscopy using a digital micromirror device

Seungwoo Shin, Doyeon Kim, and YongKeun Park
s1605 Conference on Lasers and Electro-Optics/Pacific Rim (CLEOPR) 2017

Diffraction Analysis of Digital Micromirror Device at Coherent Illumination

Zheng Xiong, Hua Liu, and Zhenwu Lu
Th14 CIOMP-OSA Summer Session on Optical Engineering, Design and Manufacturing (SumSession_OEDM) 2013

Sampling of Wavefront Aberrations using a Digital Micromirror Device

Daniya Thomas, Alessandra Carmichael Martins, and Brian Vohnsen
JTu1A.9 Frontiers in Optics (FiO) 2020

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