Abstract

This paper reports a framework for high speed volumetric imaging and orientation estimation of transparent microscopic objects using light field microscopy. The full volume of a transparent optical wing (~2.5 × 5 × 10μm) is computationally reconstructed from the recorded light field by the use of 3D deconvolution and direct volume rendering, with orientation estimated based on tensor analysis. The presented framework provides a potentiality for real time kinetic characterization of microscopic objects.

© 2016 Optical Society of America