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Hyperspectral Imaging in Live Mouse Cortex Using a 48-Channel Multiphoton Microscope

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We constructed a hyperspectral multiphoton microscope that collects 48 channels of excitation/emission spectral data, while retaining the imaging depth of nonlinear microscopy. We demonstrate imaging of multiple overlapping fluorophores in vitro and in vivo.

© 2016 Optical Society of America

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