Background Removal in Pump-Probe Microscopy

Subir Das; Bo-Wei Ho; Fu-Jen Kao

doi:10.1364/FIO.2017.FW5D.6

Frontiers in Optics 2017
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper FW5D.6
•https://doi.org/10.1364/FIO.2017.FW5D.6

Background Removal in Pump-Probe Microscopy

Subir Das, Bo-Wei Ho, and Fu-Jen Kao

Frontiers in Optics 2017

Washington, D.C. United States
18–21 September 2017
ISBN: 978-1-943580-33-0

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Advanced Microscopy and Imaging (FW5D)

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S. Das, B. Ho, and F. Kao, "Background Removal in Pump-Probe Microscopy," in Frontiers in Optics 2017, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper FW5D.6.

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Abstract

We have established a pump-probe microscopy with lock-in amplifier for the detection of spontaneous loss and stimulated gain. ATTO647N fluorescent is used for imaging. In order to separate the signal of interest from background, spontaneous loss is also detected, in addition to the stimulated gain. In this way, optical section images can be obtained with greatly reduced background, which is the origin of shot noise.

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