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Conferences > FiO > 2020 > FW5E > FW5E.5

Multiphoton Light-sheet Microscopy at Optimal Pulse Frequency for Fast In Vivo Imaging

Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, and Willy Supatto

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Abstract

Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.

© 2020 The Author(s)

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