Adaptive Optics Microscopy for Mouse Imaging

Tianyi Zheng; Kayvan F. Tehrani; A.R. Liversage; Luke J. Mortensen; Luke J. Mortensen; Peter A. Kner

doi:10.1364/FIO.2021.JTh5A.131

Frontiers in Optics + Laser Science 2021
Technical Digest Series (Optica Publishing Group, 2021),
paper JTh5A.131
•https://doi.org/10.1364/FIO.2021.JTh5A.131

Adaptive Optics Microscopy for Mouse Imaging

Tianyi Zheng, Kayvan F. Tehrani, A.R. Liversage, Luke J. Mortensen, and Peter A. Kner

Frontiers in Optics 2021

Washington, DC United States
1–4 November 2021
ISBN: 978-1-55752-308-2

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Joint Poster Session III (JTh5A)

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T. Zheng, K. F. Tehrani, A. R. Liversage, L. J. Mortensen, and P. A. Kner, "Adaptive Optics Microscopy for Mouse Imaging," in Frontiers in Optics + Laser Science 2021, C. Mazzali, T. (T.-C.) Poon, R. Averitt, and R. Kaindl, eds., Technical Digest Series (Optica Publishing Group, 2021), paper JTh5A.131.

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Abstract

We developed an AO approach to improving multiphoton microscopy in the highly distorting mouse long bone environment, which uses an SHG guidestar with SHWFS. Together with a high stroke DM, this is capable of correcting the high magnitude aberrations present in the curved sample, thereby improving the fluorescence intensity of the imaging PSF, revealing features of collagen distribution and the bone marrow environment.

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