Deep-UV-Induced Autofluorescence Spectroscopy to investigate the differential response of proteins to nonenzymatic glycation in vitro

Darshan Chikkanayakanahalli Mukunda; Kuppusamy Srinivasan Vaisshnava Laksmi; Weena Stanley; Nirmal Mazumder; Krishna Kishore Mahato

doi:10.1364/FIO.2022.JTu5A.64

Frontiers in Optics + Laser Science 2022 (FIO, LS)
Technical Digest Series (Optica Publishing Group, 2022),
paper JTu5A.64
•https://doi.org/10.1364/FIO.2022.JTu5A.64

Deep-UV-Induced Autofluorescence Spectroscopy to investigate the differential response of proteins to nonenzymatic glycation in vitro

Darshan Chikkanayakanahalli Mukunda, Kuppusamy Srinivasan Vaisshnava Laksmi, Weena Stanley, Nirmal Mazumder, and Krishna Kishore Mahato

Frontiers in Optics 2022

Rochester, New York United States
17–20 October 2022
ISBN: 978-1-957171-17-3

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Joint Poster Session IIA (In person) (JTu5A)

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D. C. Mukunda, K. S. V. Laksmi, W. Stanley, N. Mazumder, and K. K. Mahato, "Deep-UV-Induced Autofluorescence Spectroscopy to investigate the differential response of proteins to nonenzymatic glycation in vitro," in Frontiers in Optics + Laser Science 2022 (FIO, LS), Technical Digest Series (Optica Publishing Group, 2022), paper JTu5A.64.

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Abstract

Deep-UV light (280-295 nm) induced autofluorescence (AF) can be used to monitor the biochemical and structural changes in nonenzymatically glycated proteins. The current study describes the varying AF spectral signatures in Hemoglobin (Hb) and Human Serum Albumin (HSA) upon nonenzymatic glycation (NEG).

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