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Conferences > IQEC > 2009 > IMG > IMG3
  • Conference on Lasers and Electro-Optics/International Quantum Electronics Conference
  • OSA Technical Digest (CD) (Optica Publishing Group, 2009),
  • paper IMG3
  • •https://doi.org/10.1364/IQEC.2009.IMG3

Three-Dimensional Super-resolution Single-Molecule Fluorescence Imaging Using a Double-Helix Point Spread Function

Michael A. Thompson, Julie S. Biteen, W. E. Moerner, Sri Rama Prasanna Pavani, and Rafael Piestun

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Abstract

Fluorescence imaging with resolution ten times better than the diffraction limit in three dimensions over a depth of field of 2 μm is demonstrated with a widefield microscope that exhibits a double helix point spread function.

© 2009 Optical Society of America

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