Ultrafast Live 3D Imaging with 25-plane Camera Array Multifocus Microscopy

Eduardo Hirata-Miyasaki; Gustav M. Pettersson; Antone Bajor; Kaitlyn Fouke; Demis D. John; Brian Thibeault; Gal Haspel; Jennifer Morgan; Sara Abrahamsson

doi:10.1364/ISA.2022.IW1C.2

Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP)
Technical Digest Series (Optica Publishing Group, 2022),
paper IW1C.2
•https://doi.org/10.1364/ISA.2022.IW1C.2

Ultrafast Live 3D Imaging with 25-plane Camera Array Multifocus Microscopy

Eduardo Hirata-Miyasaki, Gustav M. Pettersson, Antone Bajor, Kaitlyn Fouke, Demis D. John, Brian Thibeault, Gal Haspel, Jennifer Morgan, and Sara Abrahamsson

Imaging Systems and Applications 2022

Vancouver, British Columbia Canada
11–15 July 2022
ISBN: 978-1-957171-09-8

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Super Resolution, Imaging, Microscopy II (IW1C)

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E. Hirata-Miyasaki, G. M. Pettersson, A. Bajor, K. Fouke, D. D. John, B. Thibeault, G. Haspel, J. Morgan, and S. Abrahamsson, "Ultrafast Live 3D Imaging with 25-plane Camera Array Multifocus Microscopy," in Imaging and Applied Optics Congress 2022 (3D, AOA, COSI, ISA, pcAOP), Technical Digest Series (Optica Publishing Group, 2022), paper IW1C.2.

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Abstract

We demonstrate a novel ultrafast Multifocus 25-camera-array microscope (M25) for truly simultaneous high-resolution 3D imaging of 25 focal planes. Customized for functional neural circuit imaging, our M25 microscope captures 130× 130× 50um³ volumes at >100Hz.

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