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Automated multicolour mesoscopic imaging for the 3-dimensional reconstruction of fluorescent biomarker distribution in large tissue specimens

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Abstract

We established an in-house built episcopic imaging add-on on a cryo-microtome to perform imaging during serial sectioning. This RGB and fluorescent mesoscope can provide sequential block-face and 3-dimensional anatomical images with high resolution and large field-of-view.

© 2022 The Author(s)

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