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3D Dynamic SERS Imaging of Intracellular Transport Pathways

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Abstract

Raman spectroscopy has been utilized for material analysis because Raman spectra provide information about molecular or lattice vibration of materials in a sample. Recently, Raman spectroscopy has been combined to laser scanning microscopy to image biological samples with the chemical information that cannot be detected by ordinary fluorescence techniques. However, the efficiency of Raman scattering is very weak, and therefore it is difficult to detect low-concentration molecules taking important roles for cellular functions. We have applied surface-enhanced Raman scattering (SERS), which can be observed at a surface of metal nanostructure, to enhance the detection efficiency of biological molecules participating a cellular events [1][2][3]. We introduced gold nanoparticles with a diameter of 80 nm into a HeLa cell and confirmed the strong Raman scattering from the positions that the particles exist.

© 2013 Japan Society of Applied Physics, Optical Society of America

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