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Hyperspectral holographic imaging of brain tissues using swept-source diffraction phase microscopy

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Abstract

We present hyperspectral holographic imaging of mouse brain tissues. Full-field optical amplitude and phase-delay images of a thin slice of mouse brain tissues are measured in the wavelengths ranging from 450 nm to 650 nm. To quantitatively and precisely measure the hyperspectral holographic imaging, we employed a recently develop swept-source diffraction phase microscopy [1, 2], which is composed of a custom-built wavelength-sweeping unit equipped with a supercontinuum source and diffraction phase microscopy, a common-path quantitative phase imaging system. We envision the present approach can provide a method for label-free quantification and diagnosis for biological tissue samples.

© 2014 Japan Society of Applied Physics, Optical Society of America

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