Abstract

Digital Holographic Microscopy (DHM) is a microscopic imaging technique based on interferometry. Since DHM digitally records a hologram generated by interference between object and reference beam, both amplitude and quantitative phase images can be obtained by numerically reconstructing the information of the wavefront of the light transmitted through the sample. DHM has been widely used for quantitative observation of live cell in a non-invasive way, and enables the extraction of parameters such as morphologies or dry-mass estimation, typically with picogram sensitivities by interpreting their phase as thickness [1]. As in classical wide-field microscopy, it is possible to make the measurement more specific by introducing a polarization selection in the detection scheme, such as to detect specific birefringent targets [2].

© 2015 Japan Society of Applied Physics, Optical Society of America