Abstract
Fluorescence microscope is a well-known important tool in biology for visualizing the locations of biological molecules and fluorescence nanomaterials. Chemical reactions in life processes are localized in three-dimensions in contrast to those that take place in test tubes. Therefore, to understand the spatiotemporal dynamics of biological chemical reactions, the three-dimensional (3D) mapping of points where the fluorescence originates is indispensable for analyzing biological phenomena. A self-interference digital holography can obtain the 3D fluorescence image with a small number of images without a laser scanning and a sample movement. The 3D mapping of one or several fluorescence point sources was performed [Opt. Lett. 40, 3312 (2015).].
© 2016 Japan Society of Applied Physics, Optical Society of America
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