Abstract

3-D imaging of microscopic structures with finer details is now a matured technology enriched by numerous advanced platforms like Phase-Microscopy, Stereoscopy, Holography, Depth-resolved, Angle-resolved, Differential Interference Contrast and many more [1–2]. Today nearly all the advance imaging methods demand very good quality sample preparation. This could be time consuming and may require chemical die causing the natural freshness of the sample to degrade. Keeping this in our mind, we devised a table-top experiment and demonstrated the reconstruction of 3-D surface of biological tissues through interference of high-coherence transmitted polarized lights. The experiment involves differential interference configuration of polarized light with precise alignment and stability in the measurement, which requires only a thin slice of sample on a glass slide; no other sample preparation is required.

© 2016 Japan Society of Applied Physics, Optical Society of America