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Biological Applications of UV Raman Spectroscopy: Static and Dynamical Studies

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Abstract

The recent development of UV Raman spectroscopy has led to careful studies of the enhancement mechanism of vibrational modes for both small molecules such as amides, small aromatic molecules such as substituted benzene derivatives and also larger molecules such as polycyclic aromatic hydrocarbons, DNA and proteins The objective of the small molecule studies is to characterize the resonance Raman enhancement mechanisms to examine molecular excited state structure. These studies also clarify the resonance Raman enhancement mechanisms which clarify the dependence of Raman enhancement upon molecular environment. We will present an overview of this work as it relates to ongoing UV resonance Raman studies of the heme protein structure and function and the investigation of the details of the hemoglobin cooperativity mechanism.

© 1990 Optical Society of America

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