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Conferences > LACA > 1990 > ThA > ThA5

Measurements of Rotation and Fluorescence Lifetimes for Optimizing the Detection of Single Fluorescinated Bases

Lloyd M. Davis, Newton K. Seitzinger, E. Brooks Shera, and Richard A. Keller

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Abstract

Time Correlated Single Photon Counting is an optimum technique for acquiring fluo­rescence decay profiles over a large dynamic range with accurately known errors. The data are fully utilized in a statistical sense when analyzed by weighted non-linear fitting to a decay function convolved with the experimentally measured prompt response function.1

© 1990 Optical Society of America

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