Fluorescence 3D-microscopy with 100 nm resolution

Stefan Jakobs; Alexander Egner; Stefan W. Hell

Laser Applications to Chemical and Environmental Analysis
Technical Digest (Optica Publishing Group, 2004),
paper TuB5

Fluorescence 3D-microscopy with 100 nm resolution

Stefan Jakobs, Alexander Egner, and Stefan W. Hell

Laser Applications to Chemical and Environmental Analysis 2004

Annapolis, Maryland United States
ISBN: 1-55752-765-2

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Bio-Optical I (with coffee break) (TuB)

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S. Jakobs, A. Egner, and S. W. Hell, "Fluorescence 3D-microscopy with 100 nm resolution," in Laser Applications to Chemical and Environmental Analysis, Technical Digest (Optica Publishing Group, 2004), paper TuB5.

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Abstract

Live cell fluorescence imaging of GFP labelled mitochondria of budding yeast cells using 4Pi-confocal microscopy is presented. This technique allows 3-5 times finer optical sectioning than confocal microscopy.

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