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Exploring chromatin biochemistry with single-molecule fluorescence diffusometry

Charles B. M. Limouse, Colin J. Fuller, Aaron F. Straight, and Hideo Mabuchi

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Abstract

We utilize real-time feedback to track individual dye-labeled chromatin fibers undergoing Brownian motion in aqueous buffer, enabling simultaneous recording of fluorescence and hydrodynamic data. Single-molecule experiments can be performed to study the effects of ionic concentrations and linker-histone binding on the conformation and self-association of reconstituted nucleosome arrays with defined DNA sequence and recombinant core histones.

© 2010 Optical Society of America

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