Abstract

Coherent Raman imaging methods have been under development for almost 15 years. The field is beginning to mature, transitioning from a “new techniques” phase to an applications phase, and it may now be safe to estimate the ultimate performance of these methods. I will discuss current and potential capabilities of broadband coherent anti-Stokes Raman scattering (BCARS) microscopy using optimized excitation paradigms1, and provide a few examples of how broadband BCARS imaging has helped to answer (or raise) questions in investigations of tissues and small organisms. Exemplified below, we have recently used BCARS to solve a longstanding question in adhesion of marine invertibrates to submurged substrates².

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