Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain

Masahiro Fukuda; Masahiro Fukuda; Takayoshi Matsumura; Takayoshi Matsumura; Toshio Suda; Toshio Suda; Hajime Hirase

doi:10.1364/TRANSLATIONAL.2022.JTu3A.24

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper JTu3A.24
•https://doi.org/10.1364/TRANSLATIONAL.2022.JTu3A.24

Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain

Masahiro Fukuda, Takayoshi Matsumura, Toshio Suda, and Hajime Hirase

Microscopy Histopathology and Analytics 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Joint Poster Session II Virtual (JTu3A)

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M. Fukuda, T. Matsumura, T. Suda, and H. Hirase, "Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper JTu3A.24.

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Abstract

We have developed depth-targeted Photothrombosis (DTPT) technique that can target a single vessel in the cortical parenchyma by two-photon excitation. Rose Bengal-mediated DTPT could be induced at 720 nm scanning with 84.9±1.7% success rate.

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