Non-Orthogonal Dual-Objective (NODO) Open-Top Light-Sheet (OTLS) Microscopy for 3D Pathology of Cleared Clinical Specimens

Kevin W. Bishop; Kevin W. Bishop; Adam K. Glaser; Lindsey A. Barner; Robert B. Serafin; Jonathan T.C. Liu; Jonathan T.C. Liu; Jonathan T.C. Liu

doi:10.1364/MICROSCOPY.2022.MTu4A.2

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper MTu4A.2
•https://doi.org/10.1364/MICROSCOPY.2022.MTu4A.2

Non-Orthogonal Dual-Objective (NODO) Open-Top Light-Sheet (OTLS) Microscopy for 3D Pathology of Cleared Clinical Specimens

Kevin W. Bishop, Adam K. Glaser, Lindsey A. Barner, Robert B. Serafin, and Jonathan T.C. Liu

Microscopy Histopathology and Analytics 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Novel Devices and Methods II (MTu4A)

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K. W. Bishop, A. K. Glaser, L. A. Barner, R. B. Serafin, and J. T. C. Liu, "Non-Orthogonal Dual-Objective (NODO) Open-Top Light-Sheet (OTLS) Microscopy for 3D Pathology of Cleared Clinical Specimens," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper MTu4A.2.

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Abstract

Non-orthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscopy is a novel method well-suited for moderate-NA cleared-tissue imaging. We present our latest NODO system optimized to image densely labeled clinical samples for 3D pathology.

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