Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy

Mian Wei; Lingyan Shi; Lu Wei; Wei Min; Wei Min

doi:10.1364/MICROSCOPY.2022.MTu4A.5

Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN)
Technical Digest Series (Optica Publishing Group, 2022),
paper MTu4A.5
•https://doi.org/10.1364/MICROSCOPY.2022.MTu4A.5

Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy

Mian Wei, Lingyan Shi, Lu Wei, and Wei Min

Microscopy Histopathology and Analytics 2022

Fort Lauderdale, Florida United States
24–27 April 2022
ISBN: 978-1-957171-03-6

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Novel Devices and Methods II (MTu4A)

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M. Wei, L. Shi, L. Wei, and W. Min, "Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy," in Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy, OCT, OTS, BRAIN), Technical Digest Series (Optica Publishing Group, 2022), paper MTu4A.5.

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Abstract

Raman microscopy suffers from limited imaging depth in tissues. Here we develop a volumetric chemical imaging method that combines tissue clearing with stimulated Raman scattering (SRS) microscopy, achieving greater than 10-fold depth extension.

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