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Structural 3D Imaging of Collagen-rich Tissues Using Polarimetric SHG Microscopy with Linear and Circular Polarizations

Margaux Schmeltz, Clothilde Raoux, Gaël Latour, and Marie-Claire Schanne-Klein

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Abstract

SHG microscopy is a unique tool for collagen visualization without any staining. Polarimetric approaches based on linear and circular polarizations further provide the orientation of collagen fibrils within and out of the imaging plane and their degree of disorder within the focal volume and in the field of view.

© 2022 The Author(s)

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