Abstract
We report on a novel coherent anti-Stokes Raman scattering (CARS) microscopy that significantly suppresses the nonresonant background for high contrast vibrational imaging. We employ an elliptically polarized pump field with an aspect ratio of of the ellipse combined with a linearly polarized Stokes field for elliptically polarized CARS (EP-CARS) microscopy. The EP-CARS technique yields a 6-7-fold improvement in signal-to-background ratios compared with normal CARS, while providing an approximately 1.25-fold improvement in resonant CARS signal compared with normal polarization-sensitive CARS microscopy. We demonstrate this technique by imaging 1.5 μm polystyrene beads in water.
© 2009 Optical Society of America
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