Abstract

We present experimental results on image inversion interferometric setups with significant potential to increase efficiency and resolution in fluorescence confocal microscopy. Results agree with theory in terms of a reduction of FWHM. The basic idea was described previously: The emitted image signal is sent through an interferometer in which one of the two paths inverts (rotates by 180 degrees) its image information. Constructive interference is only possible, if the emitter is right on the optic axis as both mirror images are then ideally identical. This forms the basis of the resolution improvement in a confocal setup. The designs have significant potential to increase efficiency and resolution in fluorescence confocal microscopy.

© 2009 Optical Society of America