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Remote Focusing Differential Multiphoton Microscopy: Application to Neuronal Imaging

Erich E. Hoover, Michael D. Young, Susy M. Kim, Eric V. Chandler, Jeffrey J. Field, Dawn N. Vitek, Kraig E. Sheetz, Jing W. Wang, and Jeff A. Squier

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Abstract

We apply remote focusing to multi-focal multiphoton microscopy by simultaneously imaging multiple focal planes of Drosophila melanogaster olfactory neurons. This technology permits imaging the entire volume of the antennal lobe in a single scan.

© 2011 Optical Society of America

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