Optically Sectioned Fluorescence Imaging with HiLo

Tim N. Ford; Daryl Lim; Kengyeh K. Chu; Eladio Rodriguez-Díaz; Satish K. Singh; Jerome Mertz

doi:10.1364/NTM.2011.NWB3

Optics in the Life Sciences
OSA Technical Digest (CD) (Optica Publishing Group, 2011),
paper NWB3
•https://doi.org/10.1364/NTM.2011.NWB3

Optically Sectioned Fluorescence Imaging with HiLo

Tim N. Ford, Daryl Lim, Kengyeh K. Chu, Eladio Rodriguez-Díaz, Satish K. Singh, and Jerome Mertz

Novel Techniques in Microscopy 2011

Monterey, California United States
4–6 April 2011
ISBN: 978-1-55752-909-1

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T. N. Ford, D. Lim, K. K. Chu, E. Rodriguez-Díaz, S. K. Singh, and J. Mertz, "Optically Sectioned Fluorescence Imaging with HiLo," in Optics in the Life Sciences, OSA Technical Digest (CD) (Optica Publishing Group, 2011), paper NWB3.

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Abstract

HiLo is a wide-field fluorescence imaging technique that provides optical sectioning by processing two images acquired sequentially using illumination with and without high contrast structure. We present the latest implementations of the technique.

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