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Quantitative metrics for SHG imaging of collagen-based biological structures

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Abstract

We present the application of 3D Fourier transform-second harmonic generation (3D FT-SHG) imaging to evaluate the spatial organization of collagen fibers in biological tissues. Using 3D FT-SHG, we quantified the lamellar structure in porcine sclera.

© 2013 Optical Society of America

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