Three-dimensional modes of illumination and computational reconstruction in light-sheet microscopy

George Barbastathis; George Barbastathis; Dipanjan Bhattacharya; Dipanjan Bhattacharya; Vijay R. Singh; Chen Zhi; Elijah Y. S. Yew; Peter T. C. So; Peter T. C. So; Paul Matsudaira; Yuan Luo

doi:10.1364/NTM.2013.NW1B.1

Optics in the Life Sciences
OSA Technical Digest (online) (Optica Publishing Group, 2013),
paper NW1B.1
•https://doi.org/10.1364/NTM.2013.NW1B.1

Three-dimensional modes of illumination and computational reconstruction in light-sheet microscopy

George Barbastathis, Dipanjan Bhattacharya, Vijay R. Singh, Chen Zhi, Elijah Y. S. Yew, Peter T. C. So, Paul Matsudaira, and Yuan Luo

Novel Techniques in Microscopy 2013

Waikoloa Beach, Hawaii United States
14–18 April 2013
ISBN: 978-1-55752-966-4

From the session
Phase I (NW1B)

Open Access

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G. Barbastathis, D. Bhattacharya, V. R. Singh, C. Zhi, E. Y. S. Yew, P. T. C. So, P. Matsudaira, and Y. Luo, "Three-dimensional modes of illumination and computational reconstruction in light-sheet microscopy," in Optics in the Life Sciences, OSA Technical Digest (online) (Optica Publishing Group, 2013), paper NW1B.1.

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Abstract

Three-dimensional spatial and temporal modulation of the illumination and optimal algorithms for reconstruction improve contrast from thick fluorescent tissue. Theoretical analysis and experimental results will be presented, with applications to developmental biology and live animal imaging.

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