Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Nonlinear Optical Imaging of Melanin Species using Coherent Anti-Stokes Raman Scattering (CARS) and Sum-Frequency Absorption (SFA) Microscopy

Not Accessible

Your library or personal account may give you access


Skin pigmentation correlates with melanoma incidence, and melanin subtypes are known to play key roles in melanoma pathogenesis. Here, we propose the use of CARS and SFA microscopy to selectively visualize melanins in live cells.

The annual global incidence of melanoma, the deadliest form of skin cancer, exceeds 232,000 cases worldwide, resulting in over 55,000 deaths. It has long been known that skin pigmentation strongly correlates with skin cancer incidence and, in the specific case of melanoma, carcinogenesis may in fact arise independently of ultraviolet irradiation. Indeed, the ultraviolet-radiation-independent pathway to melanoma pathogenesis is thought to be largely mediated by pheomelanin – the red/blond melanin subtype. Other studies have also shown that some resistant melanoma cell lines can overcome administration of drugs by increasing melanin production and thereby sequestering therapeutics in melanosomes, the organelles responsible for melanin production bound for cellular export. In order to further study melanoma pathogenesis and therapeutic resistance, novel imaging modalities to selectively image melanin subtypes have been developed: on one hand, coherent anti-Stokes Raman scattering (CARS) microscopy can uniquely identify pheomelanin, while on the other, sum-frequency absorption (SFA) microscopy can be used to visualize total melanin distribution.

© 2017 Optical Society of America

PDF Article
More Like This
Quantitative coherent anti-Stokes Raman scattering (CARS) microscopy of skin optical clearing dynamics

Max Zimmerley, Bernard Choi, and Eric O. Potma
BWD7 Biomedical Optics (BIOMED) 2008

New Developments in Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

Eric O. Potma, Ji-Xin Cheng, Xiaolin Nan, and X. Sunney Xie
WO3 Frontiers in Optics (FiO) 2003

Coherent Anti-Stokes Raman Scattering (CARS) for Biomedical Imaging

Conor L. Evans, Eric O. Potma, and X. Sunney Xie
LTuA3 Laser Science (LS) 2005


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription

Select as filters

Select Topics Cancel
© Copyright 2022 | Optica Publishing Group. All Rights Reserved