Widefield Polarimetric Second Harmonic Generation Techniques for Histopathology

Leonardo José Uribe Castaño; Kamdin Mirsanaye; Ahmad Golaraei; Ahmad Golaraei; Margarete K. Akens; Lukas Kontenis; Lukas Kontenis; Virginijus Barzda; Virginijus Barzda

doi:10.1364/NTM.2021.NTh1C.6

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NTh1C.6
•https://doi.org/10.1364/NTM.2021.NTh1C.6

Widefield Polarimetric Second Harmonic Generation Techniques for Histopathology

Leonardo José Uribe Castaño, Kamdin Mirsanaye, Ahmad Golaraei, Margarete K. Akens, Lukas Kontenis, and Virginijus Barzda

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Polarization and Collagen (NTh1C)

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L. J. U. Castaño, K. Mirsanaye, A. Golaraei, M. K. Akens, L. Kontenis, and V. Barzda, "Widefield Polarimetric Second Harmonic Generation Techniques for Histopathology," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NTh1C.6.

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Abstract

Measurements of different polarimetric parameters are performed using polarimetric widefield second harmonic microscopy in collagenous tissue. An analysis on the bleaching process in an H&E-stained sample is performed as well.

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