Fast holographic scattering compensation for deep tissue biological imaging

Molly A. May; Kai K. Kummer; Michaela Kress; Monika Ritsch-Marte; Alexander Jesacher

doi:10.1364/NTM.2021.NTu1C.1

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper NTu1C.1
•https://doi.org/10.1364/NTM.2021.NTu1C.1

Fast holographic scattering compensation for deep tissue biological imaging

Molly A. May, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte, and Alexander Jesacher

Novel Techniques in Microscopy 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

From the session
Phase Microscopy I (NTu1C)

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M. A. May, K. K. Kummer, M. Kress, M. Ritsch-Marte, and A. Jesacher, "Fast holographic scattering compensation for deep tissue biological imaging," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper NTu1C.1.

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Abstract

We develop a holographic phase-stepping interferometry algorithm for non-invasive scattering compensation that achieves >10x higher signal enhancement after one mode iteration than previous work and enables two-photon imaging in mouse hippocampal tissue down to 530 µm.

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