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Conferences > NTM > 2023 > NTu2C > NTu2C.3
  • Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)
  • Technical Digest Series (Optica Publishing Group, 2023),
  • paper NTu2C.3
  • •https://doi.org/10.1364/NTM.2023.NTu2C.3

Improving Autofluorescence Lifetime Decay Accuracy for Cellular Metabolic Imaging Applications

Olivia I. Kolenc, Alan E. Woessner, and Kyle P. Quinn

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Abstract

Biexponential fitting of autofluorescence lifetime decay curves is susceptible to significant error. Binning data from all pixels within whole cells and performing non-linear curvefitting in MATLAB can greatly improve measurement accuracy.

© 2023 The Author(s)

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